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Description
Step 3: The Critical Injection This is where technique is everything
The wound on the captured image was automatically recognized and evaluated via Metamorph NX image software (Molecular Devices, Sunnyvale, CA, USA), and the eluate of crystal violet (cat) staining was examined using light microscopy (EVOS FL Auto)
If you are uncomfortable with self-injecting, you can find a local clinic that will be happy to administer them for a small fee
If an unopened vial freezes, its usually okay once thawed, but you should inspect the vial carefully for any micro-cracks
doi: 10.1007/978-1-4684-5730-8_11
