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This result is compounded by the fact that -synuclein aggregation, a hallmark of PD, can also induce ferroptosis through mechanisms such as membrane disruption and altered calcium influx (53)

Cells were collected, pelleted and washed once with ice-cold 1 PBS, followed by two washes with 1 PBS + 50 mM iodoacetamine (IAA) (BioRad) with 5 min of rocking at RT for the second wash

In both case 1 and case 2, the blood tests were conducted in different hospitals

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Notably, this method enables sensitive detection of GSH at nanomolar concentrations in human serum, demonstrating strong potential for clinical diagnostic applications [93]