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For IHC, endogenous peroxidase activity was eliminated by treating the sections with 3% H2O2 for 15 minutes, following which AR (1:500, Abcam, London, UK) was incubated overnight at 4

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The peptide also appears to have some direct effects on peripheral tissues beyond the central nervous system, including potential metabolic effects, cardiovascular influences, and immunomodulatory properties

doi:10.4319/lo.1983.28.6.1275

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