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Due to its efficacy in facilitating cellular uptake and endosomal escape, which results in increased transfusion efficiency in both in vivo and in vitro, PEI has plenty of superficial positive charges and is able to condense negatively charge DNA into complexes that generate positive charge [26,27]

10.1126/science.292.5522.1728 Peng XD, Xu PZ, Chen ML, Hahn-Windgassen A, Skeen J, Jacobs J, Sundararajan D, Chen WS, Crawford SE, Coleman KG, Hay N: Dwarfism, impaired skin development, skeletal muscle atrophy, delayed bone development, and impeded adipogenesis in mice lacking Akt1 and Akt2

Occludin, claudin, and zonula occludens-1 (ZO-1) are intercellular junction proteins, essential for maintaining BRB integrity, and the breakdown of the BRB is closely linked to the dysfunction of these junction-associated proteins

doi: 10.1016/0006-8993(94)90013-2