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[DOI] [PubMed] [Google Scholar] 41.Bates SH, Stearns WH, Dundon TA, Schubert M, Tso AW, Wang Y, Banks AS, Lavery HJ, Haq AK, Maratos-Flier E, Neel BG, Schwartz MW, Myers MG., Jr STAT3 signalling is required for leptin regulation of energy balance but not reproduction

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doi: 10.1155/2011/572634

Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change (Xia et al., 2017)