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Afterwards, 3 L of diluted cDNA, 6 L of SYBR green master mix, 0.5 L of 10 mM reverse and forward primers and 2.5 L of nuclease-free water were combined for each reaction to determine gene expression by quantitative PCR (Q-PCR), which was carried out using the 7500 Real-Time PCR System (Applied Biosystems)
doi:10.1016/j.jnutbio.2005.05.013
Gln metabolism can maintain prostate cancer stem cells (CSCs) through -KG-dependent chromatin dioxygenase
Nicotinamide N -oxidation by CYP2E1 in human liver microsomes
