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Sample: 4.6 50 mm, 2.7 m The temperature was 40C

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Inset CBB-stained SDS-PAGE gel (10%) of fractions outlined on the chromatogram (45 L of 0.5 mL fraction loaded), with the primary UV absorbance from the Sr35 resistosome eluting at the 12.5-13 mL volume (filled arrows) and the AvrSr35 homodimer at the 15-15.5 mL volume (unfilled arrows)