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Preparations of recombinant proteins pGEX-PGRMC1 wt, Y113F or C129S mutant expression vectors were transformed into BL21 (DE3), and the bacteria were incubated in LB with ampicillin at 37 C until OD600 reached at 0.8

doi: 10.1016/j.cell.2017.09.021 23 WangYBiRQuanFCaoQLinYYueCet al

2022;14(6):a040246

Clinical longitudinal standards for height, weight, height velocity, weight velocity, and stages of puberty