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The HL60 cells were transfected with CHAC-targeting shRNA lentiviral particles (Sh-CHAC2) or control shRNA lentiviral particles (Sh-NC) using Lipofectamine 3000 (Invitrogen) according to the manufacturers instructions

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Valenza C, Mongillo M, Visconti MV, Katrini J, Trapani D, Boldrini L, et al

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AMPK even helps control the aging process itself by inducing several known longevity factors (such as SIRT1) that have been shown to extend lifespan in many organisms (Salminen 2011)

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