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Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity

Acknowledgements Figures were created with Biorender.com

doi: 10.3390/cells7070084

7 , e0166 (2023)

Methods Cell culture and transfection Human cervical adenocarcinoma cells (HeLaM cells were a gift from Roger Tsien, University of California, San Diego) were cultured in RPMI-1640, retinal pigment epithelial (hTERT-RPE1 purchased from ATCC, CRL-4000) cells in 1:1 mixture of DMEM and F12 HAM Media and A431 cells (purchased from the European Collection of Authenticated Cell cultures, 85090402) in DMEM