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(2021) 33:24152427.e6
A 20-minute NuCalm session is like a 1-hour nap
Therefore, those proteins that have previously been identified as a result of non-specific binding to agarose beads 25,26 were removed from the list of identified proteins in supplemental Table S1
Briefly, 3 mL of ascites was stirred with 0.6 mL of n -butanol (Sigma, 34867) and 2.4 mL of di-isopropyl ether (Sigma, 296856) for 30 min followed by centrifugation at 4,000 g at 4 C for 15 min
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