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In brief, using Fiji/ImageJ software, the raw image was split into three channels as an RGB stack, with the green channel selected for analysis due to its high contrast
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Distribution of Anesthesia Sciatic nerve block results in anesthesia of the skin of the pos-terior aspect of the thigh, hamstrings, and biceps muscles, part of the hip and knee joints, and the entire leg below the knee, with the exception of the skin of the medial aspect of the lower leg (see Figure 6 )
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