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It causes little irritation

Indocyanine green (ICG) and porphyrin are among the most widely used single-optical active molecules, exhibiting strong potential for combined PTT and PDT applications

Cayman, 36209) and 20 mM of the transpeptidation acceptor glycyl-glycine (Sigma-Aldrich, G1002) in Tris-HCl 100 mM, pH 8, buffer

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Then youll equilibrate your beads in the binding buffer, or the same buffer that youll load your GST-fusion protein onto the glutathione beads, as well discuss in the next section
