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19 Once APAP is overdosed, a large amount of NAPQI accumulates, thereby resulting in a rapid depletion of GSH in the liver

doi:10.1155/2011/879716

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This cellular alteration further raises doubts about the efficacy and reproducibility of stem cell therapy, particularly when isolating stem cells from elderly patients for regenerative purposes

Specic methods are cyanogen bromide method, reductive amination, hydroxysuccinimide technique, and carbonyldiimidazole method.N- Cyanogen Bromide Method