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Control culture variables that affect signaling: Passage number, confluence, serum concentration, extracellular matrix coating, oxygen condition, mycoplasma status, baseline cell health, and image-analysis thresholds can all influence FAK phosphorylation, VEGFR2 readouts, and tube-formation results

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This microenviroment, produced by the activation of platelets, represents an advantageous environment for EMT and EndoMT and may directly promote fibrogenesis [28] (Fig