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No reducing buffer was added to samples when probing for the lipoic arm to minimize the potential perturbation of thiol modifications on proteins

Methylglyoxal undergoes spontaneous thiolation with GSH, followed by the Glo-1 catalyzed conversion of methylglyoxal thioacetal to (S)-lactoylglutathione [232,233]

Bonini MG, Stadler K, Silva SO, Corbett J, Dore M, Petranka J, et al

247 In animal models, Wnt/-catenin signaling is reportedly attenuated during cerebral I/R, leading to increased BBB permeability and damage due to the downregulation of TJ protein in microvascular ECs

On all subjects, the following tests were performed: ECG, Holter-ECG monitoring, echocardiography and general ultrasound, cardiology consultation, usual biochemistry analysis, lipidogram and oxidative stress testing, by determining GPx values