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In addition, mass spectrometry analysis with electrospray ionisation revealed that the intracellular concentration of ZIL was 10 4 10 3 % (w/v) after 2 h of cultivation in 5% (w/v) ZIL solution, while the intracellular DMSO concentration was reported to be approximately 1.6% (w/v) after 2 h of cultivation in 7.8% (w/v) DMSO solution 14 , indicating that ZIL is a non-cell-permeable reagent

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