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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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CITY OF AREQUIPA The city of Arequipa is the most populated city after Lima, it is known as the white city of Arequipa, which is hidden in the village of Nuestra Seora de la Asunta

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