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Description
Recombinant proteins were analyzed using 10% SDS-PAGE and purified using HisTrap HP Crude Columns (GE Health) and AmiconUltra-15 Centrifugal Filter Units with Ultracel-3 membranes (Merck Millipore)

In the second step, conjugating enzymes perform addition reactions (e.g., acetyl, methyl, glucuronic acid) on these newly modified, electrophilic substrates
Trans -()-Kusunokinin binding to AKR1B1 inhibits oxidative stress and proteins involved in migration in aggressive breast cancer

Rare but serious side effects may include severe abdominal pain, persistent vomiting, allergic reactions, or signs of gallbladder or pancreatic inflammation
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