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Cells were washed with PBS, detached with trypsin and centrifuged (5 min, 400 g), resuspended and covered with 5 M working solution of MitoSOX reagent in HBSS and incubated at 37 C for 10 min in FACS tubes

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EVs have been shown to have a key role in mediating intercellular communication by carrying a variety of bioactive molecules, surface receptors, and genetic information

Practical, non-protocol fundamentals that support the healing environment Sleep consistency (regular wake time, minimized late-night light exposure, reduced alcohol)

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