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For measuring G q signaling, a plasmid mixture consisting of 200 ng LgBiT-inserted G q subunit, 500 ng G1, 500 ng C68S-mutant SmBiT-fused G2 (C68S), 200 ng cholinesterase-8A (Ric-8A) and 400 ng test GPCR in 200 L of Opti-MEM (Gibco) was transfected into cells using polyethylenimine (PEI)

The GATM and GAMT mutation-associated disorders are inherited as autosomal recessive diseases

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