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The quantification standard curve was obtained ranging from 6 nM to 20 M

As always, patient education, informed consent, rigorous monitoring and a conservative, evidence-based approach are essential

Since the 1960s it has been known that NAG is an enzyme present in kidney tissues,38 in the lysosomes of the proximal tubular cells, and that its excretion in urine increases when cells are damaged and NAG is releases into the tubular lumen .39 Classically, the determination of NAG was used to evaluate the nephrotoxicity caused by aminoglycoside antibiotics.40 Moreover, NAG levels may be increased in cases of elevated intratubular pressure as in urinary tract obstruction41 and in VUR, although in this case, as occurs with albuminuria, it is difficult to know if it is due to the elevated pressure or to the associated reduction of glomerular filtration.36 On basic determinations of kidney function as markers of parenchyma loss In a study including 77 children with normal renal parenchyma as confirmed by dimercaptosuccinic acid scan and 102 children with loss of parenchyma (one or more scars, single kidney, hypodysplasia), we determined the quality and diagnostic efficiency of the kidney function markers listed above

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The choice of timing should therefore prioritise consistency and individual tolerance rather than attempting to optimise absorption through scheduling