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Description
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left lung, fixed in 4% PFA (4 C, 24 h), cryoprotected in 30% sucrose and 30% sucrose/50% OCT (48 h each), embedded in OCT, sectioned (7 m) and stained for Dct and Grpr mRNA (RNAscope, BioTechne

Succurro E, Andreozzi F, Sciacqua A, Hribal ML, Perticone F, Sesti G

After washing with PBS (3 5 min), sections were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) for 2 h at RT protected from light

This approach extends to our use of peptides and other therapeutic options
