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After DNase I treatment, the soluble fraction was mixed with Dynabeads Protein G (Life Technologies) coupled with mouse monoclonal anti-Pk antibody (BioRad) and incubated at 4 C for 2 h

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Macrophages In murine peritoneal macrophages RSL3 blocks pro-inflammatory responses upon lipopolysaccharide treatment by inducing NRF2, which inhibits the recruitment of RNA polymerase II to the transcription start site of several cytokines [330]