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Cells were washed with PBS and lysed by freeze-thaw method (x3) in GPX4 reaction buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 1 mM EDTA, 0.1 mM DFO

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Importantly, these models often integrate both up- and down-regulated compounds, emphasizing the diagnostic value of relative metabolic balance over absolute concentration shifts

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