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Description
Neutrophil diversity and function in health and disease
Reconstitute the 1mg lyophilized vial in 10mM hydrochloric acid (HCl) at a concentration of 0.5-1.0 mg/mL to ensure complete solubilization, as IGF-1 LR3's isoelectric point near pH 8.4 makes it poorly soluble in neutral buffers and prone to aggregation at physiological pH
& Hahm, K
After staining, the media was removed from cells, cells were washed three times with PBS, and scraped into 300 L of FACS buffer
SAH: S-adenosyl-L-homocysteine
