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Mass spectrometric analysis was performed using a QTRAP 5000 triple quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source (Applied Biosystems, USA)

80 In many lineages, NAD and NAMPT may be necessary not only for proliferation but also for cell fate decisions

Any growth curves where either the mutant strain or wildtype strain biological replicates were not consistent were deemed inconclusive

However, Wangs study showed that approximately 85% of breast cancer cells have normal Rb status, but due to the rare Rb deletion in ER+ breast cancer, it is less sensitive as a predictive marker [79]