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Esterbauer H, Schaur RJ, Zollner H

The effectiveness of PDI knockdown was assessed by determining the change in cellular PDI protein levels

Reverse transcription reaction conditions were 42C, 30 minutes
1,4 Additional sensory manifestations include a feeling of walking on pins and needles, altered pain threshold, and changes in temperature sensitivity

Then, cells were extracted and washed three times, followed by centrifugation at 4 , 5000 rpm for 10 min
