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To validate these results with an orthogonal approach, we manipulated individual serotonergic neurons and tested the resulting strains on the three bacterial diets

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However, this expansion was less extensive, typically limited to regions just outside of the rostral boundary established in Unoperated Control animals

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The oligonucleotide primers were shown as follows: Hif3 (sense: 5-TCGTGCATTCTCATGAGCCC-3, antisense: 5-GTTCCTGGGCTGGACAGTTT-3), Mmp8 (sense: 5-CCAATGCCTTCCCAGTACCTGA-3, antisesce: 5-GGCATTCCTCGAAGACCGGAAT-3), Timp1 (sense: 5- CTATAGTGCTGGCTGTGGGG-3, antisense: 5-GGACCTGATCCGTCCACAAA-3), Fam187b (sense: 5 ACCAACCAGCCGTACTTTGT3, antisense: 5-TTGTCAGCTTCCCAGTGGAC-3)