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Often, they reflect expected medication effects or transient physiological changes

Ghosh A, Gupta A, Jena S, Kirti A, Choudhury A, Saha U, et al

Phil Trans R Soc B 363:31373146 Vanderschuren LJ, Kalivas PW (2000) Alterations in dopaminergic and glutamatergic transmission in the induction and expression of behavioral sensitization: a critical review of preclinical studies

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If you're not following pharmaceutical-grade sterile technique, refrigerating consistently, and discarding vials after 28 days, you're injecting expensive saline