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Variations in the expression of genes encoding key enzymes directly influence the accumulation of corresponding secondary metabolites, with PAL regulation, MEP (methylerythritol phosphate), and MVA (mevalonate) pathways seeing increased expression with MeJA treatment [36, 37]

In addition, Glu has the following features: chemical stability, metabolic generation and easy removal (interconversion with -ketoglutarate), negative charge (at physiological pH value), and acidic amino acid [due to its two carboxyl groups (- and -carboxyl) and one amino group]

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Three-month-old Tg4-42 and WT mice showed a significant preference for the target quadrant (Fig