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Blots were incubated overnight at 4 C with primary antibodies and 2 h with HRP-conjugated secondary antibodies at room temperature

Metformin, with its low cost and established safety profile, has demonstrated promising effects in improving ovarian function

Although the procedure ensures numerous benefits, it also promises many side effects if conducted without medical supervision

We then review recent advancements in understanding the mechanisms underlying ferroptosis and imbalances in iron homeostasis in human diseases