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CUMS protocol involves a variety of mild stressors and lasted for 35 days, including swimming in cold water (4C, 5 min), food deprivation for 24 h, water deprivation for 24 h, clip tail for 3 min, moist bedding (24 h), 45C cage tilting (12 h), a reversed lightdark circadian cycle (12:12 h), and restraining (6 h)

Magnesium deficiency is so rampant because certain medications, stress, and a poor diet can all deplete our bodies of magnesium

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12 13 Therapeutic Potential : In sleep medicine, modulating orexin pathways is a promising strategy for addressing conditions such as insomnia and excessive wakefulness

Therefore, the total homocysteine level in CSF may serve as a diagnostic biomarker for both PD and AD