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Drawing step by step: 1.Roll the reconstituted vial gently between your palms 5 to 10 times

The blocked membrane was incubated with 1 g/ml of either RSC or cohesin loader in 2.5% milk in TBS at 4 C overnight

This improved tolerance makes it suitable for longer-term use

The sample was mounted onto a slide glass using a mounting medium (Agilent Dako, #S3025, Santa Clara, CA, USA), and the images were captured using a confocal microscope (LSM 980, Carl Zeiss, Berlin, Germany) equipped with a 63/1.4 numerical aperture oil objective lens

[DOI] [PMC free article] [PubMed] [Google Scholar] 137.Kartha R.V., Joers J., Terluk M., Tuite P., Mishra U., Rudser K., Oz G., Weinreb N.J., Jarnes-Utz J., Cloyd J.C