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This method takes advantage of a special tag, usually a His-tag (a series of histidine residues), which binds specifically to a nickel ion immobilized on a resin

An animal model of glutathione depletion demonstrated a significant increase in oxidative stress during exercise

After washing the tissues with PBS, they were cut into pieces (1 mm1 mm) and digested with type I collagenase (Sigma, St

State Key Laboratory of Genetic Resources and Evolution, Key Laboratory of Healthy Aging Research of Yunnan Province, Kunming Key Laboratory of Healthy Aging Study, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650201, China 2