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If the scavenging capacity of the antioxidant system is insufficient, the accumulation of ROS further damages mitochondria, creating a vicious cycle

Video monitoring was used to record time to begin removing the tape, total time removing tape from first contact, and time spent actively removing tape from paws as many mice paused between removing the first and second pieces

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The assay mix, containing Assay Loading Reagent (Fluidigm), DNA Suspension Buffer (Teknova), and combined forward and reverse primers, was loaded into the assay inlets of the IFC