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Pelleted cells were lysed with lysis buffer (B-PER Bacterial Protein Extraction Reagent, ThermoFisher), lysozyme (100 g/ml), PMSF (1 mM, Sigma), protein inhibitor cocktail (ThermoFisher), DNAse (5U/ml), at room temperature for 15 min, then on ice for 15 min, then spun for 30 min

This makes it particularly effective for injuries involving connective tissues that are notoriously slow to heal on their own

Reconstituted semaglutide should be used within 28 days of mixing

Animals, orthotopic tumor implantation, and diet allocation Cell implantation Mice were anesthetized with Ketamine/Xylazine (100 mg/kg and 10 mg/kg, respectively) and assessed for lack of reflexes by toe pinch

Valkovi, L., Chmelk, M