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doi: 10.1016/j.biocel.2009.03.013

For NADPH-dependent GSSG reduction assays, 50 g of liver lysate in 10 l was mixed with 190 l of reaction mixture containing 1 mM GSSG (Sigma-Aldrich G4626) and 200 M NADPH (Sigma-Aldrich N7505) in 100 mM potassium phosphate, pH 7.0, 2 mM EDTA 10,23,41

The role of iron regulatory proteins in mammalian iron homeostasis and disease

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[DOI] [PubMed] [Google Scholar] Morgan AS, Ciaccio PJ, Tew KD and Kauvar LM