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Harvested cells were sonicated in TBS buffer (20 mM Tris pH 8.0, 150 mM NaCl) and solubilized in 200 uL of lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% DDM, 1 mM PMSF) for 2 hrs

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Co-eluting, high abundant interferences in TOFMS survey scans resulted in missed MS/MS acquisition of target peptides

PMID: 25205085