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TaqMan Gene Expression Assay (Applied Biosystems, Termo Fisher Scientific, Waltham, MA, USA) hydrolysis probes were used for gene expression analysis of selected genes ( Table 1 ) with TaqMan Gene Expression Master Mix (Applied Biosystems, Termo Fisher Scientific, Waltham, MA, USA)

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Always inspect the solution visually before administration

UF was involved in the design and conduct of the study, performed the medical supervision and contributed to the data analysis, interpretation and reporting of results