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Materials and Methods RAW264 Cells, GSH Depletion, and Treatment RAW264 cells were cultured in Roswell Park Memorial Institute medium 1640 with 2 mM l-glutamine (Sigma), 100 U/ml penicillin, 100 g/ml streptomycin sulfate (Invitrogen/Life Technologies), and 10% heat-inactivated FCS (Sigma)-omplete medium

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An illustrative reminder of the skin's structure is shown in the diagram below

The neurons were washed in PBS and then mounted in Mowiol

15 Hepatitis may develop at any age, but typically not before 2 years of age
