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Description
Further animal and human studies are needed to test IP6s antioxidants properties
(2020) 5:e135935
The protein was transferred to a polyvinylidene difluoride (PVDF) membrane by the semidry method (Bio-Rad)
For determining combinatorial treatment effects with either Reversan, MK-571, BSO or Ethacrynic acid, the medium was supplemented with the respective compound 24 h prior to assay preparation to impair transporter/enzyme function before the addition of the second drug
A 96-well microplate reader capable of reading optical absorption at 405-412 nm is required for use of this kit
