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Because of its missing SpyTag beta strand, the melting temperature of uncoupled SpyCatcher is much lower than after coupling with SpyTag 5
J Am Phys Surg 9:106108 Bowers K, Li Q, Bressler J, Avramopoulos D, Newschaffer C, Fallin MD (2011) Glutathione pathway gene variation and risk of autism spectrum disorders
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Mol Imaging Biol:95, 2005
