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Conservative protocols starting at 0.25 mg daily suit first-time users and those with minor injuries

At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)

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doi: 10.1046/j.1445-1433.2003.02682.x 14

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