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Description
Aging 33 , 588602 (2012)
Skrovanek, S., DiGuilio, K., Bailey, R., Huntington, W., Urbas, R., Mayilvaganan, B., Mercogliano, G., & Mullin, J

Blood samples from the mice were centrifuged at 300 g for 8 min at 37C, and the supernatants were measured using an alanine aminotransferase (ALT) kit (HuiLi Biotech Co., Ltd., Changchun, China) and an aspartate aminotransferase (AST) kit (Jiancheng Bioengineering Institute, Nanjing, China), according to the manufacturer's protocols
That's where Phase II steps in

Yes, the regulation of sleep is affected by many neurotransmitters and hormones
