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Samples (whole cells or isolated mitochondria) were freeze-thawed three times, and the lysates filtered through 10-kDa molecular mass cutoff devices (Amicon, Millipore) at 12,000 g for 15 min at 4 C

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In contrast, IRP2 knockdown or treatment with the ferroptosis inhibitor Fer-1 alleviates PC-12 cell damage by downregulating p53 levels and activating the SLC7A11-ALOX12 signaling pathway ( In summary, CDC42, derived from neural stem cell-derived exosomes, as well as TIGAR, FTO, and IRP2, contribute to PD pathology by regulating iron homeostasis, ferroptosis, fatty acid metabolism, the antioxidant system, and angiogenesis

[DOI] [PubMed] [Google Scholar] 34.Regeneration of static-load-degenerated articular cartilage extracellular matrix by vitamin C supplementation