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Description
After incubation for 4 h at 37 C, the steroids were extracted with chloroform, evaporated by centrifugal concentrator, and re-dissolved in methanol for liquid chromatographymass spectrometry (LCMS) analysis, using buffer A containing 1 mM ammonium formate and buffer B containing 100% methanol

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(collectively "First Trust")

doi: 10.1056/NEJMoa0907929
