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It is more and more taken into consideration that gluten does not seem to be the only cause of discomfort in wheat-sensitive patients [29]

Purification The purification procedure is similar as in 47 with the following modifications: 1) The membrane fraction was solubilized by addition of an equal volume of: 10 mM sodium phosphate (NaP i ) at pH 8, 10% glycerol, 0.1 mM EDTA, 1 mM GSH, and 6% Triton X-100 (Sigma) and followed by 45 minutes incubation in 4 C

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Reproducing complete whisker trimming in adults has a similar, albeit much smaller effect that requires prolonged deprivation (8 weeks of deprivation are required