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All the observations mentioned for the first novel mouse were also measured for the second mouse for ten minutes
A pragmatic alternative is to prepare smaller vials from your reconstituted stock and freeze them as single-use aliquots at 20C, minimizing repeated freeze-thaw exposure to your main vial
[DOI] [PMC free article] [PubMed] [Google Scholar] 155.Logozzi M., Spugnini E., Mizzoni D., Di Raimo R., Fais S
Fineman, M
After each incubation, supernatants were collected
