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Chemical Type Chemical Maximum Concentration Reducing Agents DTE 5mM DTT 20mM bME (b-mercaptoethanol) 20mM TCEP 5mM L-Glutathione, reduced 40mM Denaturing Agents Urea 8M Guanidine HCl 6M Detergents Triton X-100 (nonionic) 2% Tween 20 (nonionic) 2% NP-40 (nonionic) 2% Cholate (anionic) 2% CHAPS (zwitterionic) 1% Other Ethanol 20% Glycerol 50% Sodium Sulfate 100mM Sodium Chloride 1.5M Buffers Sodium Phosphate, pH 7.4 50mM Tris HCl, pH 7.4 100mM Tris Acetate, pH 7.4 100mM HEPES, pH 7.4 100mM MOPS, pH 7.4 100mM Keep in mind that while glutathione and glutathione agarose beads are compatible with all of these components, you need to ensure that your GST tag is folded and capable of binding to glutathione